扫描荧光相关光谱法。I. 聚集测量的理论与模拟
Scanning fluorescence correlation spectroscopy. I. Theory and simulation of aggregation measurements.
作者信息
Petersen N O
出版信息
Biophys J. 1986 Apr;49(4):809-15. doi: 10.1016/S0006-3495(86)83709-2.
Abstract
Scanning Fluorescence Correlation Spectroscopy (S-FCS) is introduced as an adaptation of Fluorescence Correlation Spectroscopy (FCS) to measure aggregation in systems, such as biological cell membranes, where diffusion or flow is slow. The theoretical framework for interpretation of S-FCS measurements are discussed in this paper with emphasis on the limitations arising from the sample size and shape. Computer simulations of the experiment demonstrate the potential of the technique and illustrate how some of the limitations may be overcome.
摘要
扫描荧光相关光谱法（S-FCS）是作为荧光相关光谱法（FCS）的一种改进方法而引入的，用于测量诸如生物细胞膜等系统中的聚集情况，在这些系统中扩散或流动较为缓慢。本文讨论了用于解释S-FCS测量结果的理论框架，重点关注了因样品大小和形状而产生的局限性。该实验的计算机模拟展示了该技术的潜力，并说明了如何克服一些局限性。
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