Petersen N O, Johnson D C, Schlesinger M J
Biophys J. 1986 Apr;49(4):817-20. doi: 10.1016/S0006-3495(86)83710-9.
Scanning fluorescence correlation spectroscopy is a new approach to measuring changes in the state of aggregation of cell membrane proteins. Measurements of the mean number of aggregates of virus glycoproteins from Sindbis virus and vesicular stomatitis virus agree with the findings of a recent fluorescence photobleaching recovery study on the same systems (Johnson, D.C., M.J. Schlesinger, and E.L. Elson, 1981, Cell, 23:423-431). Sindbis Virus glycoproteins are immobilized and cannot be induced to aggregate further by antibody cross linking. In this study, we find that Sindbis virus glycoprotein is more highly aggregated than vesicular stomatitis virus glycoprotein, which can be patched further with antibody. These measurements demonstrate the potential of scanning fluorescence correlation spectroscopy in studies of aggregation problems in membranes of cultured cells.
扫描荧光相关光谱法是一种用于测量细胞膜蛋白聚集状态变化的新方法。对辛德毕斯病毒和水疱性口炎病毒的病毒糖蛋白聚集体平均数的测量结果，与最近对相同系统进行的荧光光漂白恢复研究结果一致（约翰逊，D.C.，M.J.施莱辛格，和E.L.埃尔森，1981年，《细胞》，23:423 - 431）。辛德毕斯病毒糖蛋白是固定化的，不能通过抗体交联进一步诱导聚集。在本研究中，我们发现辛德毕斯病毒糖蛋白比水疱性口炎病毒糖蛋白的聚集程度更高，后者可以用抗体进一步形成斑片。这些测量结果证明了扫描荧光相关光谱法在研究培养细胞膜聚集问题方面的潜力。
