Springer D L, Reed D J, Dost F N
J Toxicol Environ Health. 1981 Jul-Aug;8(1-2):11-9. doi: 10.1080/15287398109530046.
A method is described for collection of metabolic 15N2 from in vitro preparations or intact rats administered 15N-containing compounds. The methods enables routine collection and mass spectrometric measurement of as little as 10 mumol 15N2 respired by a rat over a 24-h period. A device is described that includes either an animal chamber or a tissue reaction vessel in a closed recycling atmosphere, with automatic O2 replenishment and removal of CO2 and water. It is capable of sustaining moderate vacuum and is coupled to a high-vacuum manifold designed to process the contained atmosphere and respiratory gases. The starting atmosphere is an 80:20 mix of sulfur hexafluoride and O2. Recovery of 15N2 gas from the system without an animal present was 101.3 +/- 5.75%. When 15N2 gas was very slowly infused iv into an animal, recovery was 89.1 +/- 5.38%. Use of the method in studies of the fate of [15N]hydrazine in rats indicated that about 15% of the administered hydrazine is rapidly converted to 15N2, followed by slower conversion of an additional 7-10% over the next several hours.
本文描述了一种从体外制剂或给予含(^{15}N)化合物的完整大鼠中收集代谢性(^{15}N_2)的方法。该方法能够常规收集并通过质谱法测量大鼠在24小时内呼出的低至(10)微摩尔的(^{15}N_2)。文中介绍了一种装置，该装置在封闭的循环气氛中包括一个动物室或一个组织反应容器，具有自动补充氧气以及去除二氧化碳和水的功能。它能够维持适度的真空，并与一个高真空歧管相连，该歧管设计用于处理所包含的气氛和呼吸气体。起始气氛是六氟化硫和氧气的(80:20)混合物。在没有动物的情况下，从系统中回收(^{15}N_2)气体的回收率为(101.3\pm5.75%)。当将(^{15}N_2)气体非常缓慢地静脉注入动物体内时，回收率为(89.1\pm5.38%)。该方法在大鼠体内对([^{15}N])肼的代谢研究中的应用表明，所给予的肼约有(15%)迅速转化为(^{15}N_2)，随后在接下来的几个小时内，又有另外(7 - 10%)的较慢转化。
