Taylor L, Polgar P
Prostaglandins. 1981 Nov;22(5):723-8. doi: 10.1016/0090-6980(81)90211-2.
Prostaglandin (PG) synthesis was determined in human embryo lung fibroblasts (HELF) during active, slowed and non-growing phases. Bradykinin and ascorbic acid were used to induce PG synthesis. The cells were also exposed to arachidonic acid, a PG precursor. During active growth, PGE2 synthesis in response to stimulation by either bradykinin or ascorbic acid was low. As growth slowed the cellular response changed. During quiescence bradykinin and ascorbic acid stimulated PG production markedly while the conversion of free arachidonic acid to PGE2 also increased markedly. This change in response by quiescent cells was not due to an increase in cell density. When growing and quiescent cells at the same cell density were compared, the growing cells showed very little response to bradykinin while the quiescent cells were very responsive. The change in response was also not due to any difference in arachidonic acid concentrations in the culture medium during growth and non-growth.
在活跃、生长减缓及非生长阶段测定了人胚肺成纤维细胞（HELF）中前列腺素（PG）的合成。使用缓激肽和抗坏血酸诱导PG合成。细胞还暴露于PG前体花生四烯酸中。在活跃生长期间，缓激肽或抗坏血酸刺激引起的PGE2合成较低。随着生长减缓，细胞反应发生变化。在静止期，缓激肽和抗坏血酸显著刺激PG产生，同时游离花生四烯酸向PGE2的转化也显著增加。静止细胞的这种反应变化并非由于细胞密度增加所致。当比较相同细胞密度的生长细胞和静止细胞时，生长细胞对缓激肽的反应很小，而静止细胞反应非常灵敏。反应的变化也不是由于生长和非生长期间培养基中花生四烯酸浓度的任何差异引起的。
