组织培养中兔肾髓质间质细胞的前列腺素生物合成。血管紧张素II、缓激肽和精氨酸加压素的刺激作用。
Prostaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin, and arginine vasopressin.
作者信息
Zusman R M, Keiser H R
出版信息
J Clin Invest. 1977 Jul;60(1):215-23. doi: 10.1172/JCI108758.
Rabbit renomedullary interstitial cells were isolated and grown in tissue culture. These cells synthesize 0.8 ng of prostaglandin E2 (PGE2) per microgram cellular protein per hour in monolayer tissue culture; prostaglandins A2 and F2alpha (PGA2 and PGF2alpha) biosynthesis was 10 and 5% of PGE2 biosynthesis, respectively. Arachidonic acid markedly stimulated the production of PGE2 and PGF2alpha, with conversion rates of 0.24 and 0.02%/h, respectively. Angiotensin II, hyperosmolality, bradykinin, and arginine vasopressin each stimulated PGE2 biosynthesis; maximum stimulation was 20, 3.7, 3.6, and 3.2 times basal production, respectively. PGE2 biosynthesis by the renomedullary interstitial cells was inhibited by isoproterenol, potassium, nonsteroidal anti-inflammatory agents (indomethacin, naproxen, ibuprofen, suprofen, meclofenamate, and acetylsalicylic acid), mepacrine (a phospholipase inhibitor), hydrocortisone, and cortisone. The rabbit renomedullary interstitial cell in tissue culture is a model system for the study of hormonal regulation of PGE2 biosynthesis.
兔肾髓质间质细胞被分离出来并在组织培养中生长。这些细胞在单层组织培养中每微克细胞蛋白每小时合成0.8纳克前列腺素E2（PGE2）；前列腺素A2和F2α（PGA2和PGF2α）的生物合成分别为PGE2生物合成的10%和5%。花生四烯酸显著刺激PGE2和PGF2α的产生，转化率分别为0.24%/小时和0.02%/小时。血管紧张素II、高渗、缓激肽和精氨酸加压素均刺激PGE2的生物合成；最大刺激分别为基础产量的20倍、3.7倍、3.6倍和3.2倍。异丙肾上腺素、钾、非甾体抗炎药（吲哚美辛、萘普生、布洛芬、舒洛芬、甲氯芬那酸和乙酰水杨酸）、米帕林（一种磷脂酶抑制剂）、氢化可的松和可的松抑制肾髓质间质细胞的PGE2生物合成。组织培养中的兔肾髓质间质细胞是研究PGE2生物合成激素调节的模型系统。
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