Costa J L, Kirk K L, Stark H
Res Commun Chem Pathol Pharmacol. 1981 Sep;33(3):547-58.
When washed human platelets accumulate serotonin (5HT), it is possible with the use of formaldehyde fixative to measure uptake over periods as short as 10 seconds. To evaluate the utility of these short times for kinetic studies of 5HT uptake, we have examined the accuracy with which the 10 second uptake rate reflects changes in the extracellular 5HT concentration. The amount of 5HT taken up over a 10 second period appears to be determined by the concentration of 5HT present in the extracellular medium during that time period, and the uptake rate can change rapidly following sudden changes in the extracellular 5HT concentration. However, the extent of inhibition of 5HT uptake in the presence of imipramine of extracellular 5HT concentrations greater than 10-5M is somewhat different depending on whether the 5HT is allowed to accumulate for a 10-second or a 5- or 10-minute uptake period. A 10-second uptake period thus appears to be useful for kinetic studies of initial 5HT uptake velocities under normal conditions, but under inhibitory conditions may give results differing from those obtained with minute-long periods.
当洗涤过的人体血小板积累血清素（5-羟色胺，5HT）时，使用甲醛固定剂能够测量短至10秒时间段内的摄取量。为了评估这些短时间对于5HT摄取动力学研究的实用性，我们检验了10秒摄取率反映细胞外5HT浓度变化的准确性。在10秒时间段内摄取的5HT量似乎由该时间段细胞外介质中存在的5HT浓度决定，并且在细胞外5HT浓度突然变化后摄取率会迅速改变。然而，在细胞外5HT浓度大于10^-5M的情况下，存在丙咪嗪时5HT摄取的抑制程度根据5HT是积累10秒还是5分钟或10分钟摄取时间段而有所不同。因此，10秒摄取时间段似乎对于正常条件下初始5HT摄取速度的动力学研究有用，但在抑制条件下可能给出与长达数分钟时间段所获得的结果不同的结果。
