Wennersten G
Acta Derm Venereol. 1977;57(6):519-24.
The potential phototoxic capacity of some metal compounds of clinical significance in dermatology has been investigated by means of the photohemolysis technique. No photosensitized hemolysis of erythrocytes was observed with the chromium, nickel, copper and cobalt compounds studied. On the contrary, nickel and cobalt compounds proved to be efficient in quenching singlet oxygen mediated photo-oxidative membrane damage of red blood cells. Cadmium compounds showed a pronounced photohemolytic activity and the reaction was oxygen dependent. The deuterium test for singlet oxygen showed a significant increase in photohemolytic efficiency. The addition of histidine, a known quencher of singlet oxygen, resulted in a significant inhibition of the photohemolysis. Pronounced photo-oxidative damage to plasma membranes was also observed in vitro cultivated cells by means of scanning electron microscopy.
通过光溶血技术研究了皮肤科临床中一些具有重要意义的金属化合物的潜在光毒性。在所研究的铬、镍、铜和钴化合物中，未观察到红细胞的光致敏溶血现象。相反，镍和钴化合物被证明能有效淬灭单线态氧介导的红细胞光氧化膜损伤。镉化合物表现出明显的光溶血活性，且该反应依赖于氧气。单线态氧的氘测试表明光溶血效率显著提高。添加已知的单线态氧淬灭剂组氨酸会导致光溶血受到显著抑制。通过扫描电子显微镜还在体外培养细胞中观察到对质膜的明显光氧化损伤。
