Fukuda H, Iritani N
J Biochem. 1981 Dec;90(6):1757-62. doi: 10.1093/oxfordjournals.jbchem.a133653.
After the intraperitoneal administration of radioactive cholic acid to rats, the binding of the cholic acid to protein was immunologically investigated. The serum and liver cytosol were separately incubated with antiligandin or antialbumin immunoglobulin and then subjected to sucrose density gradient centrifugation. The sedimentation behavior of the radioactivity, endogenous bile acids, glutathione S-transferase activity of ligandin and protein provided evidence that cholic acid is bound to both ligandin and albumin in liver, and mostly to albumin in serum. However, little, if any, cholic acid seems to be bound to protein in bile. These results suggest that ligandin and albumin are the major physiological carriers of bile acid.
给大鼠腹腔注射放射性胆酸后，对胆酸与蛋白质的结合进行了免疫学研究。将血清和肝细胞溶质分别与抗配体蛋白或抗白蛋白免疫球蛋白孵育，然后进行蔗糖密度梯度离心。放射性、内源性胆汁酸、配体蛋白的谷胱甘肽S-转移酶活性以及蛋白质的沉降行为提供了证据，表明胆酸在肝脏中与配体蛋白和白蛋白都有结合，而在血清中主要与白蛋白结合。然而，胆汁中似乎很少（如果有的话）胆酸与蛋白质结合。这些结果表明，配体蛋白和白蛋白是胆汁酸的主要生理载体。
