小鼠肝脏、肾脏、骨骼和胎盘碱性磷酸酶的Akp-2基因座遗传学。与4号染色体上的Ahd-1基因座连锁。
Genetics of the Akp-2 locus for alkaline phosphatase of liver, kidney, bone, and placenta in the mouse. Linkage with the Ahd-1 locus on chromosome 4.
作者信息
Wilcox F H, Taylor B A
出版信息
J Hered. 1981 Nov-Dec;72(6):387-90. doi: 10.1093/oxfordjournals.jhered.a109537.
Differences between strains of mice were observed in electrophoretic migration on cellulose acetate plates of alkaline phosphatase in butanol extracts of kidney, bone and placenta. Each strain exhibited a broad band with fast or slow migration. Distribution among strains including recombinant inbred (Rl) strains indicated the variation in these organs to be the same as the previously described in the liver as coded for by the Akp-2 locus. An analysis of Rl strains for the kidney isozyme showed 43 with the faster moving form (AKP-2A), and 43 with the slower moving form (AKP-2B), and thus in the 1:1 ratio expected if a single locus is responsible for the strain differences. Comparisons of Rl strains showed linkage of the Akp-2 locus to two loci on chromosome 4 as follows: centromere-Gpd-1-5.7 +/- 1.9 cM-Akp-2-0.6 +/- 0.4 cM-Ahd-1. These data based on 82 Rl strains typed for both the first and second loci, and 86 for the second and third loci.
在醋酸纤维素板上对肾脏、骨骼和胎盘丁醇提取物中的碱性磷酸酶进行电泳迁移时，观察到了不同品系小鼠之间的差异。每个品系都呈现出一条迁移速度快或慢的宽带。包括重组近交（Rl）品系在内的各品系间的分布情况表明，这些器官中的变异与之前在肝脏中所描述的相同，由Akp - 2位点编码。对肾脏同工酶的Rl品系分析显示，43个品系具有迁移速度较快的形式（AKP - 2A），43个品系具有迁移速度较慢的形式（AKP - 2B），因此，如果单个位点负责品系差异，预期比例为1:1。对Rl品系的比较表明，Akp - 2位点与4号染色体上的两个位点连锁，如下所示：着丝粒 - Gpd - 1 - 5.7 +/- 1.9 cM - Akp - 2 - 0.6 +/- 0.4 cM - Ahd - 1。这些数据基于82个Rl品系对第一个和第二个位点进行分型，以及86个品系对第二个和第三个位点进行分型。
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