Distribution of Fc gamma receptors on isolated gut enterocytes of the suckling rat.

An erythrocyte-antibody rosette assay has been used to study the presence and distribution of Fc gamma receptors on enterocytes isolated from 12-day-old suckling rat gut by means of a buffer medium containing EGTA. Such receptors were found to be restricted to enterocytes in the proximal region (duodenum and jejunum) of the small intestine and to be acid-pH dependent. For the majority of enterocytes indicator red cells bound in high density to the abluminal plasmalemma but not to the apical microvillous brush border. Since immunofluorescence studies revealed strong binding of added IgG to the microvillous region, a likely explanation is that there is a paucity of Fc gamma receptors from the tips of microvilli (at least under the conditions of the rosette assay) and that receptors more deeply situated as inaccessible to indicator red cells. Binding of indicator red cells was readily inhibited by rabbit, human, guinea pig and rat IgG but less so by bovine IgG, and of the two sub-classes, bovine IgG2 inhibited much more readily than bovine IgG1. Cortisone acetate injection virtually abolished Fc gamma receptor expression on isolated enterocytes within three days. These findings correlate both with selective transport of IgG of different species in vivo and the known effect of cortisone acetate to terminate such transport.