Sági J, Szabolcs A, Szemzö A, Otvös L
Nucleic Acids Res. 1981 Dec 21;9(24):6985-94. doi: 10.1093/nar/9.24.6985.
Time course of incorporation and the effect of 5'-triphosphate of the selective antiherpetic agent (E)-5-(2-bromovinyl)-2'-deoxyuridine (bv5dUTP) on the incorporation of dTTP and dATP into template-primers of different structure were studied in E. coli DNA polymerase I Klenow fragment enzyme-catalyzed reactions. bv5dUTP could substitute for dTTP depending on the structure of template-primer. E.g. into calf thymus DNA incorporation of bv5dUMP was around 80% of that of dTMP at 30 minutes of incubation. The analog has also inhibited dTMP incorporation, net DNA synthesis, however, was hardly affected. The substrate properties of the analog were studied with [2-14C]-labelled bv5dUTP.
在大肠杆菌DNA聚合酶I Klenow片段酶催化反应中,研究了选择性抗疱疹剂(E)-5-(2-溴乙烯基)-2'-脱氧尿苷(bv5dUTP)的掺入时间进程及其5'-三磷酸对dTTP和dATP掺入不同结构模板引物的影响。bv5dUTP可以根据模板引物的结构替代dTTP。例如,在孵育30分钟时,bv5dUMP掺入小牛胸腺DNA的量约为dTMP的80%。该类似物也抑制了dTMP的掺入,然而,净DNA合成几乎没有受到影响。用[2-14C]标记的bv5dUTP研究了该类似物的底物性质。