修饰的多核苷酸。I. 5-烷基-dUTP的酶促聚合反应研究
Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s.
作者信息
Sági J T, Szabolcs A, Szemzö A, Otvös L
出版信息
Nucleic Acids Res. 1977 Aug;4(8):2767-77. doi: 10.1093/nar/4.8.2767.
Abstract
The chemical synthesis of 5-alkyl-dUTP-s and their participation as substrates in poly[d(A-6)] primed polymerization reactions with dATP by E. coli DNA polymerase I enzyme has been described. In comparison with dTTP, at saturating substrate concentrations, the rate of hypochromic effect was found to be 17.3% higher for dUTP and was lower by 27.4% for 5-ethyl-dUTP, 29.5% for 5-n-propyl-dUTP, 31.4% for 5-n-butyl-dUTP and by 85.0% for 5-n-pentyl-dUTP. No hypochromic effect could be observed, however, with 5-iso-propyl-, 5-tert.butyl- and 5-n-hexyl-dUTP-s. Polydeoxynucleotides have also been isolated from the reaction mixture and some of their structural properties determined.
摘要
已报道了5-烷基-dUTP的化学合成及其作为底物参与大肠杆菌DNA聚合酶I以聚[d(A-6)]为引物、dATP进行的聚合反应。与dTTP相比，在底物浓度饱和时，发现dUTP的减色效应速率比dTTP高17.3%，5-乙基-dUTP低27.4%，5-正丙基-dUTP低29.5%，5-正丁基-dUTP低31.4%，5-正戊基-dUTP低85.0%。然而，5-异丙基-、5-叔丁基-和5-正己基-dUTP未观察到减色效应。还从反应混合物中分离出了聚脱氧核苷酸，并测定了它们的一些结构性质。
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