Sági J, Szabolcs A, Szemźó A, Otvös L
Nucleic Acids Res. 1986 Apr 25;14(8):3449-62. doi: 10.1093/nar/14.8.3449.
5-Isopropyl-2'-deoxyuridine (ip5dU) was recently recognized as a clinically useful antiherpetic (HSV-1) agent. An ip5dU-containing polynucleotide, poly (dA-dT, ip5dU) was prepared to study how physical and bio-organic properties of the synthetic DNA model poly (dA-dT) would change upon partial substitution of thymidine. Synthesis was carried out with DNA polymerase enzyme and the polymers contained 7-9% of ip5dU. It proved to be less thermostable than poly (dA-dT) and the transition width was highly increased. Although it was a very efficient template for DNA polymerase enzyme, template activity for RNA synthesis was strongly reduced by the presence of ip5dU. Diminished stability against enzymic degradation, especially against single-strand-specific Nuclease S1 was also observed.
5-异丙基-2'-脱氧尿苷（ip5dU）最近被认为是一种临床上有用的抗疱疹（HSV-1）药物。制备了一种含ip5dU的多核苷酸聚（dA-dT，ip5dU），以研究合成DNA模型聚（dA-dT）的物理和生物有机性质在胸腺嘧啶部分取代后会如何变化。用DNA聚合酶进行合成，聚合物中含有7-9%的ip5dU。结果证明它的热稳定性比聚（dA-dT）差，且转变宽度大幅增加。尽管它是DNA聚合酶的高效模板，但ip5dU的存在会强烈降低其对RNA合成的模板活性。还观察到其对酶促降解的稳定性降低，尤其是对单链特异性核酸酶S1的稳定性降低。
