修饰多核苷酸。VI. 一种含有抗疱疹剂（E）-5-（2-溴乙烯基）-2'-脱氧尿苷的合成DNA的性质
Modified polynucleotides. VI. Properties of a synthetic DNA containing the anti-herpes agent (E)-5-(2-bromovinyl)-2'-deoxyuridine.
作者信息
Sági J, Czuppon A, Kajtár M, Szabolcs A, Szemzö A, Otvös L
出版信息
Nucleic Acids Res. 1982 Oct 11;10(19):6051-66. doi: 10.1093/nar/10.19.6051.
Abstract
A new modified polydeoxynucleotide, a copolymer of nucleotides of 2'-deoxyadenosine and the very efficacious anti-herpesvirus agent (E)-5-(2-bromovinyl)-2'-deoxyuridine was synthesized with E. coli DNA polymerase I enzyme. It is characterized by its physical (absorption and circular dichroism spectra, thermal transition, sedimentation analysis) and bioorganic (template activity, stability) properties. Compared to poly [d(A-T)], the modified polydeoxynucleotide had a lower thermal stability but exhibited higher stability against DNases and higher template activity for DNA synthesis. Template activity for RNA synthesis of this template was, however, poor and extent of AMP and UMP incorporation was limited as well.
摘要
一种新的修饰多脱氧核苷酸，即2'-脱氧腺苷核苷酸与非常有效的抗疱疹病毒剂（E）-5-（2-溴乙烯基）-2'-脱氧尿苷的共聚物，使用大肠杆菌DNA聚合酶I酶合成。它具有物理性质（吸收光谱和圆二色光谱、热转变、沉降分析）和生物有机性质（模板活性、稳定性）。与聚[d（A-T）]相比，修饰的多脱氧核苷酸热稳定性较低，但对脱氧核糖核酸酶表现出更高的稳定性，并且对DNA合成具有更高的模板活性。然而，该模板的RNA合成模板活性较差，AMP和UMP掺入程度也有限。
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HELIX--RANDOM COIL TRANSITIONS IN DNA HOMOPOLYMER PAIRS.DNA同聚物对中的螺旋-无规卷曲转变
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