Micu D, Guran M, Olinescu V, Constantineanu I
Med Interne. 1981 Oct-Dec;19(4):397-404.
To estimate the degree of lymphocyte reactivity in patients with internal diseases with immune components, the authors used a new method of investigation, the area of lymphocyte nucleoli (ALN), recently proposed by them. ALN was determined in blood cultures stimulated with phytohemagglutinin (PHA) and sacrificed after three days. The blood was obtained from 120 patients with chronic internal diseases with immune components such as: bronchial asthma, chronic hepatitis, rheumatoid arthritis, systemic lupus erythematosus or chronic lymphocytic leukemia (CLL). The data obtained showed clearly that, compared with the classical test of lymphocyte blastic transformation, ALN--which presented increases even by more than 100% over normal in rheumatoid arthritis and in systemic lupus erythematosus--can supply additional information regarding the degree of reactivity of the cultivated lymphocytes. In CLL, as it was to be expected, ALN values were below the normal.
为评估患有免疫相关内科疾病患者的淋巴细胞反应程度，作者采用了一种新的研究方法——淋巴细胞核仁面积（ALN），这是他们近期提出的方法。ALN是在经植物血凝素（PHA）刺激并在三天后处死的血液培养物中测定的。血液取自120例患有免疫相关慢性内科疾病的患者，这些疾病包括：支气管哮喘、慢性肝炎、类风湿关节炎、系统性红斑狼疮或慢性淋巴细胞白血病（CLL）。所获数据清楚表明，与淋巴细胞母细胞转化的经典检测相比，ALN在类风湿关节炎和系统性红斑狼疮中甚至比正常水平增加超过100%，它能够提供有关培养淋巴细胞反应程度的额外信息。在CLL中，正如预期的那样，ALN值低于正常水平。
