Tinggaard Pedersen N, Cohn J
Scand J Haematol. 1981 Jul;27(1):57-63. doi: 10.1111/j.1600-0609.1981.tb00452.x.
A total of 110 children, aged 0-15 years, were investigated for circulating megakaryocytes in cubital venous blood using the saponin-haemolysis leucoconcentration technique. The average number of megakaryocytes decreased from 17.8 per ml blood in the first year of life to 5.5 after the 6th year, which is the same value as in adult humans. The intact thrombocytogenic megakaryocyte value decreases from about 40% in the first year of life to only a few percent (less than 5%) after the 10th year, the same value as in adult humans. There was a significantly higher number of megakaryocytes in children aged 0-6 years than in those aged 7-15 years. We concluded that occurrence of intact megakaryocytes greater than or equal to 25% in the venous blood is a sign of a normal thrombopoietic activity in the bone marrow, and the percentage of intact megakaryocytes in cubital venous blood reflects the decrease in thrombopoiesis in the bone marrow of fingers, hands and forearms during childhood. The decline in thrombopoietic activity is concentrated in three periods: 1-3, 6-7, and 10-11 years. An occurrence of intact megakaryocytes greater than 5% in venous blood draining organs or bone marrow is a sign of some thrombopoietic activity.
采用皂素溶血白细胞富集技术，对110名年龄在0至15岁的儿童肘静脉血中的循环巨核细胞进行了研究。巨核细胞的平均数量从出生后第一年每毫升血液中的17.8个降至6岁后的5.5个，这与成年人的数值相同。完整的血小板生成巨核细胞值从出生后第一年的约40%降至10岁后的仅百分之几（小于5%），这与成年人的数值相同。0至6岁儿童的巨核细胞数量明显高于7至15岁的儿童。我们得出结论，静脉血中完整巨核细胞的发生率大于或等于25%是骨髓正常血小板生成活动的标志，肘静脉血中完整巨核细胞的百分比反映了儿童期手指、手部和前臂骨髓中血小板生成的减少。血小板生成活动的下降集中在三个时期：1至3岁、6至7岁和10至11岁。引流器官或骨髓的静脉血中完整巨核细胞的发生率大于5%是一些血小板生成活动的标志。
