通过从抹香鲸肌红蛋白已知的抗原结构进行推断，对人血红蛋白中的两个抗原位点进行合成预测和验证。
Prediction and conformation by synthesis of two antigenic sites in human haemoglobin by extrapolation from the known antigenic structure of sperm-whale myoglobin.
作者信息
Kazim A L, Atassi M Z
出版信息
Biochem J. 1977 Oct 1;167(1):275-8. doi: 10.1042/bj1670275.
Abstract
The complete antigenic structure of sperm-whale myoglobin was previously determined in our laboratory. By structural analogy with myoglobin, two regions in human haemoglobin were predicted to comprise antigenic sites. One region was on the alpha-chain [alpha-(15-23)] and the other on the beta-chain [beta-(16-23)]. These two regions were synthesized, purified and characterized, and their immunochemistry was studied. Each peptide was able specifically to bind considerable amounts of haemoglobin antibodies. In a set of homologous proteins, barring any drastic conformational or electrostatic inductive effects exerted by the substitutions, and allowing for obstruction due to subunit interaction, the determination of the antigenic structure of one protein may serve as a useful starting model for the others.
摘要
抹香鲸肌红蛋白完整的抗原结构先前已在我们实验室测定。通过与肌红蛋白的结构类比，预测人血红蛋白中的两个区域包含抗原位点。一个区域位于α链[α-(15 - 23)]，另一个位于β链[β-(16 - 23)]。合成、纯化并表征了这两个区域，并研究了它们的免疫化学性质。每种肽都能特异性结合大量的血红蛋白抗体。在一组同源蛋白质中，排除由于取代作用产生的任何剧烈构象或静电诱导效应，并考虑到亚基相互作用导致的阻碍，一种蛋白质抗原结构的测定可为其他蛋白质提供有用的起始模型。
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1
Prediction and conformation by synthesis of two antigenic sites in human haemoglobin by extrapolation from the known antigenic structure of sperm-whale myoglobin.通过从抹香鲸肌红蛋白已知的抗原结构进行推断，对人血红蛋白中的两个抗原位点进行合成预测和验证。
Biochem J. 1977 Oct 1;167(1):275-8. doi: 10.1042/bj1670275.
2
The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species.对肌红蛋白的抗体反应与免疫物种无关。分析了在不同物种中产生的十五种肌红蛋白与抹香鲸肌红蛋白抗血清交叉反应的抗原位点和环境残基中的替换情况。
Biochem J. 1980 Dec 1;191(3):681-97. doi: 10.1042/bj1910681.
3
First consequences of the determination of the entire antigenic structure of sperm-whale myoglobin.抹香鲸肌红蛋白完整抗原结构测定的初步结果。
Adv Exp Med Biol. 1978;98:19-40. doi: 10.1007/978-1-4615-8858-0_3.
4
Antibodies against protein antigenic sites that are identical in the homologous protein of the immunized animal. Autoreactivity in rabbits of antibodies to sperm-whale myoglobin.针对免疫动物同源蛋白中相同蛋白质抗原位点的抗体。兔体内抗抹香鲸肌红蛋白抗体的自身反应性。
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Immunochemistry of sperm whale myoglobin. I. The specific interaction of some tryptic peptides and of peptides containing all the reactive regions of the antigen.抹香鲸肌红蛋白的免疫化学。I. 一些胰蛋白酶肽段以及包含抗原所有反应区域的肽段的特异性相互作用。
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