Kazim A L, Atassi M Z
Biochem J. 1977 Oct 1;167(1):275-8. doi: 10.1042/bj1670275.
The complete antigenic structure of sperm-whale myoglobin was previously determined in our laboratory. By structural analogy with myoglobin, two regions in human haemoglobin were predicted to comprise antigenic sites. One region was on the alpha-chain [alpha-(15-23)] and the other on the beta-chain [beta-(16-23)]. These two regions were synthesized, purified and characterized, and their immunochemistry was studied. Each peptide was able specifically to bind considerable amounts of haemoglobin antibodies. In a set of homologous proteins, barring any drastic conformational or electrostatic inductive effects exerted by the substitutions, and allowing for obstruction due to subunit interaction, the determination of the antigenic structure of one protein may serve as a useful starting model for the others.
抹香鲸肌红蛋白完整的抗原结构先前已在我们实验室测定。通过与肌红蛋白的结构类比,预测人血红蛋白中的两个区域包含抗原位点。一个区域位于α链[α-(15 - 23)],另一个位于β链[β-(16 - 23)]。合成、纯化并表征了这两个区域,并研究了它们的免疫化学性质。每种肽都能特异性结合大量的血红蛋白抗体。在一组同源蛋白质中,排除由于取代作用产生的任何剧烈构象或静电诱导效应,并考虑到亚基相互作用导致的阻碍,一种蛋白质抗原结构的测定可为其他蛋白质提供有用的起始模型。