Kazim A L, Atassi M Z
Biochem J. 1980 Jan 1;185(1):285-7. doi: 10.1042/bj1850285.
Haptoglobin binding to haemoglobin and its isolated alpha- and beta-chains was studied by use of a highly sensitive solid-phase radiometric assay. As expected, adsorbents of haemoglobin bound 125I-labelled haptoglobin more efficiently than did adsorbents of the alpha-chain. However, unexpectedly, adsorbents of the beta-chain were found to be essentially identical with those of the alpha-chain in their ability to bind haptoglobin. These results demonstrate, unequivocally, the ability of beta-chains to bind to haptoglobin, and indicate that this assay is particularly convenient and useful for studying haptoglobin interactions with haemoglobin and its alpha- and beta-chains.
利用高灵敏度的固相放射分析方法研究了触珠蛋白与血红蛋白及其分离的α链和β链的结合情况。正如预期的那样，血红蛋白吸附剂比α链吸附剂更有效地结合125I标记的触珠蛋白。然而，出乎意料的是，发现β链吸附剂在结合触珠蛋白的能力上与α链吸附剂基本相同。这些结果明确地证明了β链与触珠蛋白结合的能力，并表明该分析方法对于研究触珠蛋白与血红蛋白及其α链和β链的相互作用特别方便且有用。
