Dohoo I R, McMillan I, Meek A H
Can J Comp Med. 1981 Oct;45(4):335-8.
Fresh milk samples and potassium dichromate preserved milk samples were stored at both ambient, approximately 21 degree C, and refrigerator temperatures, 3-5 degree C, for varying lengths of time before somatic cell counts were performed on an electronic particle counter. Fresh milk samples stored at ambient temperatures became unacceptable for somatic cell counting by 16 hours while those stored in the refrigerator were acceptable for up to three days. Once dichromate had been added to the milk no difference in cell counts attributable to temperature of storage were detected and there was very little change with time up to 14 days. On the average the addition of the dichromate elevated the cell counts/mL. As well a method of rapid fixation of milk involving the addition of glutaraldehyde prior to counting was evaluated. In fresh milk samples the use of glutaraldehyde as a fixative required adjustment of the threshold setting on the cell counter in order to produce results comparable to those obtained from formalin fixed samples. With dichromate preserved milk samples, glutaraldehyde fixation generally elevated the cell counts but the results were variable.
新鲜牛奶样本和重铬酸钾保存的牛奶样本分别在环境温度（约21摄氏度）和冷藏温度（3 - 5摄氏度）下储存不同时长，然后使用电子粒子计数器进行体细胞计数。储存在环境温度下的新鲜牛奶样本在16小时后就不适合进行体细胞计数了，而储存在冰箱中的样本在长达三天的时间内都适合计数。一旦向牛奶中添加了重铬酸钾，未检测到储存温度对细胞计数有差异，并且在长达14天的时间内变化很小。平均而言，添加重铬酸钾会使每毫升细胞计数升高。此外，还评估了一种在计数前添加戊二醛的快速固定牛奶的方法。在新鲜牛奶样本中，使用戊二醛作为固定剂需要调整细胞计数器的阈值设置，以便产生与福尔马林固定样本相当的结果。对于重铬酸钾保存的牛奶样本，戊二醛固定通常会提高细胞计数，但结果不稳定。
