Greer D O, Pearson J K
J Dairy Res. 1976 Oct;43(3):371-9. doi: 10.1017/s0022029900015958.
This paper is a sequel to a previous one in which a number of factors likely to influence the accuracy of counting somatic cells in milk was assessed; in the present work the effects of 5 other factors are investigated. In a study of storage time and temperature of milk samples fixed in formalin, a significant increase in cell count occurred after 5-7 d when samples were stored at room temperature (17-23 degrees C), compared with those maintained at 4 degrees C. When manual and mechanical mixing of fixed samples were compared only marginal differences in cell counts were observed. An increase in cell counts followed manual dilution of milk samples in comparison with automatic dilution. The temperature of samples prepared for counting was also studied and no significant variations occurred between mean temperatures of 12-7 and 32-9 degrees C. The final factor evaluated was that of holding time before counting; using 4 cell-count levels it was observed that counts were acceptable up to 1 1/2 h.
本文是前文的续篇，前文评估了一些可能影响牛奶体细胞计数准确性的因素；在本研究中，对另外5个因素的影响进行了调查。在一项关于福尔马林固定的牛奶样本储存时间和温度的研究中，与保存在4℃的样本相比，当样本在室温（17-23℃）下储存5-7天后，细胞计数显著增加。当比较固定样本的手动和机械混合时，仅观察到细胞计数的微小差异。与自动稀释相比，手动稀释牛奶样本后细胞计数增加。还研究了用于计数的样本温度，在12-7℃和32-9℃的平均温度之间未发现显著差异。评估的最后一个因素是计数前的保存时间；使用4个细胞计数水平，观察到计数在1个半小时内是可接受的。
