Semeraro N, Colucci M, Mussoni L, Donati M B
Agents Actions. 1981 Dec;11(6-7):646-8. doi: 10.1007/BF01978778.
We have investigated the ability of rat and rabbit leucocytes to generate coagulant activity (PCA) in response to endotoxin in vitro and in vivo. On prolonged incubation with endotoxin (10 microgram/ml f.c.) isolated rabbit leukocytes developed strong PCA as measured by clotting and amidolytic assay. In contrast, rat leucocyte failed to produce any PCA even in the presence of huge amounts of endotoxin (200 microgram/ml f.c.) When rabbits were given two spaced endotoxin injections (25 microgram/kg b.w., 24 h apart) blood leucocytes harvested 30--60 min after the second injection consistently showed marked PCA. Again, unlike injections (up 2 mg/kg b.w.) were completely devoid of PCA. These findings support the view that leucocytes are involved in endotoxin-induced disseminated intravascular coagulation in rabbits. On the other hand the poor response of rat leucocytes to endotoxin might help explain the resistance of rats to DIC and Sanarelli-Shwartzman reaction.
我们研究了大鼠和家兔白细胞在体外和体内对内毒素产生凝血活性（PCA）的能力。用内毒素（10微克/毫升，腹腔注射）长时间孵育后，通过凝血和酰胺水解测定法测量，分离出的家兔白细胞产生了很强的PCA。相比之下，即使存在大量内毒素（200微克/毫升，腹腔注射），大鼠白细胞也未能产生任何PCA。当家兔接受两次间隔的内毒素注射（25微克/千克体重，间隔24小时）时，在第二次注射后30 - 60分钟采集的血液白细胞始终显示出明显的PCA。同样，与家兔不同，大鼠注射高达2毫克/千克体重的内毒素后完全没有PCA。这些发现支持了白细胞参与家兔内毒素诱导的弥散性血管内凝血的观点。另一方面，大鼠白细胞对内毒素的反应较差可能有助于解释大鼠对弥散性血管内凝血和施瓦茨曼反应的抵抗力。
