Niemetz J
J Clin Invest. 1972 Feb;51(2):307-13. doi: 10.1172/JCI106815.
Peritoneal leukocytes harvested from rabbits which have received two spaced doses of endotoxin have significantly greater (10-fold) coagulant activity than leukocytes from control rabbits. The coagulant activity accelerates the clotting of normal plasma and activates factor X in the presence of factor VII and calcium and is therefore regarded as tissue factor. A total of 40-80 mg tissue factor activity was obtained from the peritoneal cavity of single endotoxin-treated rabbits. In leukocyte subcellular fractions, separated by centrifugation, the specific tissue factor activity sedimented mainly at 14,500 g and above. The procoagulant activity was destroyed after heating for 10 min at 65 degrees C but was preserved at lower temperatures. Polymyxin B, when given with the first dose of endotoxin, reduced both the number of peritoneal leukocytes and their tissue factor activity by two-thirds. When given immediately before the second dose of endotoxin, polymyxin B had no inhibitory effect.
从接受两剂间隔一定时间的内毒素的兔子体内采集的腹膜白细胞，其凝血活性比对照兔子的白细胞显著更高（10倍）。这种凝血活性能加速正常血浆的凝固，并在因子VII和钙存在的情况下激活因子X，因此被视为组织因子。从单次接受内毒素处理的兔子腹腔中可获得总共40 - 80毫克的组织因子活性。在通过离心分离的白细胞亚细胞组分中，特定的组织因子活性主要沉淀在14,500克及以上。凝血活性在65摄氏度加热10分钟后被破坏，但在较低温度下得以保留。多粘菌素B在给予第一剂内毒素时，可使腹膜白细胞数量及其组织因子活性减少三分之二。在第二剂内毒素给药前立即给予多粘菌素B，则没有抑制作用。
