α-肾上腺素能介导的Ca2+流入心脏线粒体的激活。一种调节线粒体内游离Ca2+的可能机制。
The alpha-adrenergic-mediated activation of Ca2+ influx into cardiac mitochondria. A possible mechanism for the regulation of intramitochondrial free CA2+.
作者信息
Kessar P, Crompton M
出版信息
Biochem J. 1981 Nov 15;200(2):379-88. doi: 10.1042/bj2000379.
Abstract
Mitochondria isolated from rat hearts perfused with adrenaline, and from hearts excised from adrenaline-treated rats, showed an enhanced rate of respiration-dependent Ca2+ uptake. Adrenaline pretreatment did not change the activity of the Na+/Ca2+-antiporter of isolated heart mitochondria. Simultaneous measurements of the membrane potential revealed that perfusion with adrenaline has no significant effect on this parameter during Ca2+ accumulation. The activation of Ca2+ uptake was induced also by the alpha-adrenergic agonist, methoxamine, but not by the beta-adrenergic agonist, isoprenaline. Methoxamine pretreatment also increased the sensitivity of alpha-oxoglutarate dehydrogenase in intact mitochondria to 10 nM--300 nM extramitochondrial Ca2+ during steady-state Ca2+ recycling across the inner membrane. Possible implications of these data for the adrenergic regulation of oxidative metabolism are discussed.
摘要
从用肾上腺素灌注的大鼠心脏以及从经肾上腺素处理的大鼠身上切除的心脏中分离出的线粒体，显示出呼吸依赖性Ca2+摄取速率增强。肾上腺素预处理并未改变分离的心脏线粒体中Na+/Ca2+反向转运体的活性。同时测量膜电位发现，在Ca2+积累过程中，用肾上腺素灌注对该参数没有显著影响。α-肾上腺素能激动剂甲氧明也可诱导Ca2+摄取的激活，但β-肾上腺素能激动剂异丙肾上腺素则不能。在稳态Ca2+跨内膜循环过程中，甲氧明预处理还增加了完整线粒体中α-酮戊二酸脱氢酶对10 nM - 300 nM线粒体外Ca2+的敏感性。讨论了这些数据对肾上腺素能调节氧化代谢的可能影响。
相似文献
1
The alpha-adrenergic-mediated activation of Ca2+ influx into cardiac mitochondria. A possible mechanism for the regulation of intramitochondrial free CA2+.α-肾上腺素能介导的Ca2+流入心脏线粒体的激活。一种调节线粒体内游离Ca2+的可能机制。
Biochem J. 1981 Nov 15;200(2):379-88. doi: 10.1042/bj2000379.
2
The alpha-adrenergic-mediated activation of the cardiac mitochondrial Ca2+ uniporter and its role in the control of intramitochondrial Ca2+ in vivo.α-肾上腺素能介导的心脏线粒体钙离子单向转运体的激活及其在体内线粒体钙调控中的作用。
Biochem J. 1983 Nov 15;216(2):333-42. doi: 10.1042/bj2160333.
3
Role of Ca2+ ions in the regulation of intramitochondrial metabolism in rat heart. Evidence from studies with isolated mitochondria that adrenaline activates the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes by increasing the intramitochondrial concentration of Ca2+.钙离子在大鼠心脏线粒体内代谢调节中的作用。来自对分离线粒体研究的证据表明，肾上腺素通过增加线粒体内钙离子浓度来激活丙酮酸脱氢酶和2-氧代戊二酸脱氢酶复合物。
Biochem J. 1984 Feb 15;218(1):235-47. doi: 10.1042/bj2180235.
4
Effects of adrenergic agonists and mitochondrial energy state on the Ca2+ transport systems of mitochondria.肾上腺素能激动剂和线粒体能量状态对线粒体Ca2+转运系统的影响。
Biochemistry. 1987 Jan 13;26(1):246-54. doi: 10.1021/bi00375a034.
5
Role of calcium ions in the regulation of intramitochondrial metabolism. Effects of Na+, Mg2+ and ruthenium red on the Ca2+-stimulated oxidation of oxoglutarate and on pyruvate dehydrogenase activity in intact rat heart mitochondria.钙离子在调节线粒体内代谢中的作用。钠离子、镁离子和钌红对完整大鼠心脏线粒体中钙离子刺激的草酰戊二酸氧化及丙酮酸脱氢酶活性的影响。
Biochem J. 1980 Jul 15;190(1):107-17. doi: 10.1042/bj1900107.
6
Evidence for beta-adrenergic activation of Na+-dependent efflux of Ca2+ from isolated liver mitochondria.β-肾上腺素能激活离体肝线粒体中Na⁺依赖的Ca²⁺外流的证据。
Biochem J. 1982 Apr 15;204(1):369-71. doi: 10.1042/bj2040369.
7
Glycogen phosphorylase activation by two different alpha 1-adrenergic receptor subtypes: methoxamine selectively stimulates a putative alpha 1-adrenergic receptor subtype (alpha 1a) that couples with Ca2+ influx.两种不同的α1 - 肾上腺素能受体亚型激活糖原磷酸化酶：甲氧明选择性刺激一种与Ca2+内流偶联的假定α1 - 肾上腺素能受体亚型（α1a）。
Mol Pharmacol. 1989 Jul;36(1):166-76.
8
Studies on the interactions of Ca2+ and pyruvate in the regulation of rat heart pyruvate dehydrogenase activity. Effects of starvation and diabetes.关于Ca2+与丙酮酸在调节大鼠心脏丙酮酸脱氢酶活性中的相互作用的研究。饥饿和糖尿病的影响。
Biochem J. 1982 Feb 15;202(2):419-27. doi: 10.1042/bj2020419.
9
The activation of Na+-dependent efflux of Ca2+ from liver mitochondria by glucagon and beta-adrenergic agonists.胰高血糖素和β-肾上腺素能激动剂对肝脏线粒体中Na⁺依赖性Ca²⁺外流的激活作用。
Biochem J. 1983 Feb 15;210(2):463-72. doi: 10.1042/bj2100463.
10
Evidence that adrenaline activates key oxidative enzymes in rat liver by increasing intramitochondrial [Ca2+].有证据表明，肾上腺素通过增加线粒体内[Ca2+]来激活大鼠肝脏中的关键氧化酶。
FEBS Lett. 1985 Jan 28;180(2):259-64. doi: 10.1016/0014-5793(85)81082-6.
引用本文的文献
1
Ca2+ cycling between sarcoplasmic reticulum and mitochondria in rabbit cardiac myocytes.兔心肌细胞肌浆网与线粒体之间的钙离子循环
J Physiol. 1993 Jan;460:603-21. doi: 10.1113/jphysiol.1993.sp019489.
2
Evidence for beta-adrenergic activation of Na+-dependent efflux of Ca2+ from isolated liver mitochondria.β-肾上腺素能激活离体肝线粒体中Na⁺依赖的Ca²⁺外流的证据。
Biochem J. 1982 Apr 15;204(1):369-71. doi: 10.1042/bj2040369.
3
Evidence for the existence of regulatory sites for Ca2+ on the Na+/Ca2+ carrier of cardiac mitochondria.心脏线粒体Na+/Ca2+载体上存在Ca2+调节位点的证据。
Biochem J. 1982 Feb 15;202(2):509-18. doi: 10.1042/bj2020509.
4
The alpha-adrenergic-mediated activation of the cardiac mitochondrial Ca2+ uniporter and its role in the control of intramitochondrial Ca2+ in vivo.α-肾上腺素能介导的心脏线粒体钙离子单向转运体的激活及其在体内线粒体钙调控中的作用。
Biochem J. 1983 Nov 15;216(2):333-42. doi: 10.1042/bj2160333.
5
Mitochondrial Ca2+ transport in lean and genetically obese (ob/ob) mice.瘦小鼠和遗传性肥胖（ob/ob）小鼠中的线粒体钙转运
Biochem J. 1983 Jul 15;214(1):163-70. doi: 10.1042/bj2140163.
6
The isolation of lymphocyte mitochondria and their regulation of extramitochondrial free Ca2+ concentration.淋巴细胞线粒体的分离及其对外线粒体游离钙离子浓度的调节。
Biochem J. 1982 Mar 15;202(3):731-7. doi: 10.1042/bj2020731.
7
Decreased flux through pyruvate dehydrogenase during calcium ion movements induced by vasopressin, alpha-adrenergic agonists and the ionophore A23187 in perfused rat liver.在灌注大鼠肝脏中，由血管加压素、α-肾上腺素能激动剂和离子载体A23187诱导的钙离子移动过程中，丙酮酸脱氢酶通量降低。
Biochem J. 1983 May 15;212(2):271-8. doi: 10.1042/bj2120271.
8
The activation of Na+-dependent efflux of Ca2+ from liver mitochondria by glucagon and beta-adrenergic agonists.胰高血糖素和β-肾上腺素能激动剂对肝脏线粒体中Na⁺依赖性Ca²⁺外流的激活作用。
Biochem J. 1983 Feb 15;210(2):463-72. doi: 10.1042/bj2100463.
9
The effect of alpha-adrenergic agonists on the membrane potential of fat-cell mitochondria in situ.α-肾上腺素能激动剂对原位脂肪细胞线粒体膜电位的影响。
Biochem J. 1982 Sep 15;206(3):619-26. doi: 10.1042/bj2060619.
10
Studies on the activation of rat liver pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase by adrenaline and glucagon. Role of increases in intramitochondrial Ca2+ concentration.肾上腺素和胰高血糖素对大鼠肝脏丙酮酸脱氢酶和2-氧代戊二酸脱氢酶的激活作用研究。线粒体内钙离子浓度升高的作用。
Biochem J. 1985 Nov 1;231(3):597-608. doi: 10.1042/bj2310597.
本文引用的文献
1
2
Calcium transport in mitochondria.线粒体中的钙转运
FEBS Lett. 1970 Sep 18;10(1):1-5. doi: 10.1016/0014-5793(70)80402-1.
3
Stable changes to calcium fluxes in mitochondria isolated from rat livers perfused with alpha-adrenergic agonists and with glucagon.在用α-肾上腺素能激动剂和胰高血糖素灌注的大鼠肝脏中分离出的线粒体中，钙通量发生稳定变化。
Biochem J. 1980 May 15;188(2):443-50. doi: 10.1042/bj1880443.
4
The regulation of brain mitochondrial calcium-ion transport. The role of ATP in the discrimination between kinetic and membrane-potential-dependent calcium-ion efflux mechanisms.脑线粒体钙离子转运的调节。ATP在区分动力学和膜电位依赖性钙离子外流机制中的作用。
Biochem J. 1980 Mar 15;186(3):833-9. doi: 10.1042/bj1860833.
5
The activation of pyruvate dehydrogenase in the perfused rat heart by adrenaline and other inotropic agents.肾上腺素及其他变力性药物对灌注大鼠心脏中丙酮酸脱氢酶的激活作用。
Biochem J. 1981 Feb 15;194(2):639-43. doi: 10.1042/bj1940639.
6
On the role of the calcium transport cycle in heart and other mammalian mitochondria.论钙转运循环在心脏及其他哺乳动物线粒体中的作用。
FEBS Lett. 1980 Sep 22;119(1):1-8. doi: 10.1016/0014-5793(80)80986-0.
7
Mitochondrial calcium transport.线粒体钙转运
FEBS Lett. 1980 Mar 10;111(2):261-8. doi: 10.1016/0014-5793(80)80806-4.
8
Ca2+-regulated release of an ATPase inhibitor protein from submitochondrial particles derived from skeletal muscles of the rat.
J Biol Chem. 1980 Jan 10;255(1):267-73.
9
The sodium ion/calcium ion cycle of cardiac mitochondria.心脏线粒体的钠离子/钙离子循环
Biochem Soc Trans. 1980 Jun;8(3):261-2. doi: 10.1042/bst0080261.
10
Role of calcium ions in the regulation of intramitochondrial metabolism. Effects of Na+, Mg2+ and ruthenium red on the Ca2+-stimulated oxidation of oxoglutarate and on pyruvate dehydrogenase activity in intact rat heart mitochondria.钙离子在调节线粒体内代谢中的作用。钠离子、镁离子和钌红对完整大鼠心脏线粒体中钙离子刺激的草酰戊二酸氧化及丙酮酸脱氢酶活性的影响。
Biochem J. 1980 Jul 15;190(1):107-17. doi: 10.1042/bj1900107.
Zotero 插件