Bhalla S C, Narang S
Can J Genet Cytol. 1981;23(4):705-13. doi: 10.1139/g81-076.
Zymograms of leucine-amino-peptidase (LAP) of the mosquito Culex pipiens fatigans Wiedemann (C. p. quinquefasciatus) showed 20 isozyme bands in the fourth instar larvae, 13 in the pupae and 3 in the older adults. Homozygous strains of Lap-1F (Fast) and Lap-1S (Slow) were isolated and linkage studies carried out. Lap-1F and Lap-1S allozymes act as codominant alleles, are sex linked and are located in linkage group I. The gene sequence is e-M-mar-Lap-1 (eye-gap, maroon-eye, leucine-amino-peptidase-1), and the linkage distances in the M-mar and mar-Lap-1 segments are 22.0 and 10.5, respectively. Gene frequency analysis of natural populations from Brazil and Pakistan showed the S allele was more frequent in the Brazilian populations whereas F was more frequent in the Pakistani populations. Fixation of F allele had occurred in two Pakistani populations.
致倦库蚊（Culex pipiens fatigans Wiedemann，即C. p. quinquefasciatus）的亮氨酸氨基肽酶（LAP）酶谱显示，四龄幼虫中有20条同工酶带，蛹中有13条，老龄成虫中有3条。分离出了Lap-1F（快）和Lap-1S（慢）的纯合菌株，并进行了连锁研究。Lap-1F和Lap-1S同工酶作为共显性等位基因，是性连锁的，位于连锁群I中。基因序列为e-M-mar-Lap-1（眼间隙、栗色眼、亮氨酸氨基肽酶-1），M-mar和mar-Lap-1片段中的连锁距离分别为22.0和10.5。对来自巴西和巴基斯坦的自然种群进行的基因频率分析表明，S等位基因在巴西种群中更常见，而F等位基因在巴基斯坦种群中更常见。在两个巴基斯坦种群中发生了F等位基因的固定。
