Petersen I, Ladefoged P
Dev Biol Stand. 1981;50:141-4.
In order to further reduce the amount of monkeys used for production of inactivated poliovaccine without expensive investments, cultivation of second and third passage cells from Cercopithecus monkey kidneys in monolayer cultures has been attempted. Steep growth curves were obtained for all three passages. But the initial loss of cells in the first passage was a problem. Another one was the increased risk of contamination when higher passages of monolayer cultures were used. For practical purposes it may therefore be more favourable to use second instead of third passage cells.
为了在不进行昂贵投资的情况下进一步减少用于生产灭活脊髓灰质炎疫苗的猴子数量，已尝试在单层培养中培养来自猕猴肾脏的第二代和第三代传代细胞。所有三代传代细胞均获得了陡峭的生长曲线。但第一代传代细胞最初的损失是个问题。另一个问题是使用更高代次的单层培养物时污染风险增加。因此，出于实际目的，使用第二代而非第三代传代细胞可能更有利。
