Edevåg G, Grandien M, Mares I
J Virol Methods. 1985 Aug;11(4):347-55. doi: 10.1016/0166-0934(85)90028-x.
The exclusion of SV40 contamination in poliovaccine produced in Cynomolgus monkey kidney cell cultures is routinely done by inoculation of the inactivated vaccine into Cercopithecus monkey kidney cell cultures where a cytopathic effect reveals the presence of the virus. An ELISA is described for the detection of SV40 antigen and the efficiency of antigen detection was compared with the development of cytopathic effect in Cercopithecus tissue cultures. The assay shortened considerably the time for production control and was in full agreement with the conventional test method.
通过将灭活疫苗接种到猕猴肾细胞培养物中来常规排除食蟹猴肾细胞培养物中生产的脊髓灰质炎疫苗中的SV40污染，在这种培养物中，细胞病变效应可揭示病毒的存在。本文描述了一种用于检测SV40抗原的酶联免疫吸附测定法，并将抗原检测效率与食蟹猴组织培养物中细胞病变效应的发展进行了比较。该测定法大大缩短了生产控制时间，并且与传统测试方法完全一致。
