Dahling D R, Wright B A
Appl Environ Microbiol. 1986 Apr;51(4):790-812. doi: 10.1128/aem.51.4.790-812.1986.
An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, sera, NaHCO3 levels, culture bottles, cell concentration, overlay media, agar, virus infection conditions, and cell-dissociating agents. Additionally, a comparative virus isolation study with BGM cells and nine other cell types was conducted with 37 sewage samples collected from nine different geographic areas. The results of the study indicated that the BGM cell line is superior for virus isolation when compared with the other cell types and that certain media and additives tend to increase BGM cell sensitivity to a specific group of viruses. A standardized procedure for cultivation of BGM cells is described which provides a more effective enterovirus assay system.
本文描述了对指定为BGM的连续细胞系的深入研究，并就细胞培养和病毒检测程序的标准化提出了建议。根据对使用该细胞系的58个实验室的调查收集的数据，制定了一项研究计划，其中包括对生长培养基、血清、碳酸氢钠水平、培养瓶、细胞浓度、覆盖培养基、琼脂、病毒感染条件和细胞解离剂的研究。此外，还对从九个不同地理区域收集的37份污水样本进行了BGM细胞与其他九种细胞类型的比较病毒分离研究。研究结果表明，与其他细胞类型相比，BGM细胞系在病毒分离方面更具优势，并且某些培养基和添加剂倾向于提高BGM细胞对特定病毒组的敏感性。本文描述了一种标准化的BGM细胞培养程序，该程序提供了一种更有效的肠道病毒检测系统。
