Low-molecular-weight zinc-binding proteins in liver cytosol of the allylisopropylacetamide (AIA) treated rats.

Rats maintained on a normal diet supplemented with 10 microCi 65Zn/day for a period of 10 days were given allylisopropylacetamide (AIA), 400 mg/kg body weight: 24 h later they were killed. Cytosol was prepared from liver cells and analysed by gel filtration on a Sephadex G-75 column. The AIA treatment caused an alteration in the chromatographic pattern of Zn-binding proteins, and a low-molecular-weight fraction appeared.