Gapparov M M, Liapkov B G
Biokhimiia. 1980 Oct;45(10):1744-8.
A protein fraction possessing a 209 times higher lipid-carrying activity in comparison with the original supernatant (X 105 000 g) was isolated from the livers of Wistar rats. The purification procedure included precipitation at pH 5,1, desalting with ammonium sulfate and gel-filtration through Sephadex G-50. Evidence from polyacrylamide gel electrophoresis suggest that the fraction obtained consists of two components with molecular weights of 18 000 and 30 000. The half-life time of the proteins as determined by double radioactive labelling is 3,7 days. The microsomal oxidation inducer phenobarbital increases the half-life time of lipid-carrying proteins by decreasing their degradation rate.
从Wistar大鼠肝脏中分离出一种蛋白质组分，其脂质携带活性比原始上清液（105000g离心）高209倍。纯化过程包括在pH 5.1下沉淀、硫酸铵脱盐以及通过Sephadex G-50进行凝胶过滤。聚丙烯酰胺凝胶电泳的结果表明，得到的组分由分子量分别为18000和30000的两种成分组成。通过双重放射性标记测定，这些蛋白质的半衰期为3.7天。微粒体氧化诱导剂苯巴比妥通过降低脂质携带蛋白的降解速率来延长其半衰期。
