离体工作大鼠心脏中的三羧酸循环通量及酶活性
Tricarboxylic acid cycle flux and enzyme activities in the isolated working rat heart.
作者信息
Cooney G J, Taegtmeyer H, Newsholme E A
出版信息
Biochem J. 1981 Dec 15;200(3):701-3. doi: 10.1042/bj2000701.
Abstract
Flux through the tricarboxylic acid cycle was calculated from oxygen consumption in hearts perfused near the physiological work load. Activities of citrate synthase, 2-oxoglutarate dehydrogenase and succinate dehydrogenase were measured in the same hearts. Only the activities of 2-oxoglutarate dehydrogenase correlated with calculated fluxes through the cycle.
摘要
通过三羧酸循环的通量是根据在接近生理工作负荷下灌注的心脏中的耗氧量计算得出的。在同一心脏中测量了柠檬酸合酶、2-氧代戊二酸脱氢酶和琥珀酸脱氢酶的活性。只有2-氧代戊二酸脱氢酶的活性与通过该循环计算出的通量相关。
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