Snider T G, Adams L G, Pierce K R
Vet Immunol Immunopathol. 1981 Apr;2(2):175-88. doi: 10.1016/0165-2427(81)90048-9.
Nine of seventeen neonatal Holstein-Friesian calves were thymectomized, treated with antilymphocyte globulin, and monitored for immunologic functional ability for 4 to 6 months. The thymus weights for 4 to 10-day-old calves and 4 to 6-month-old calves indicated a continued increase in total weight. This indicated significant thymic involution had not occurred at 4 to 6 months. Following thymectomy a wasting syndrome was not observed although an increased incidence of a lowly virulent virus infection did occur. A significant decrease in circulating lymphocytes was observed. Peripheral blood lymphocytes were stimulated in vitro by non-specific mitogens, phytohemagglutinin, bacterial lipopolysaccharide and pokeweed mitogen using the whole blood culture method. Observations included a greater response to phytohemagglutinin and pokeweed mitogen in summer months and variable age related response to all mitogens. Lipopolysaccharide stimulation results were inconclusive. It was concluded that neonatal thymectomy was not a satisfactory experimental procedure for the production of selective immunosuppression in the bovine species.
17头新生荷斯坦-弗里生犊牛中的9头接受了胸腺切除术，并用抗淋巴细胞球蛋白进行治疗，并对其免疫功能进行了4至6个月的监测。4至10日龄犊牛和4至6月龄犊牛的胸腺重量表明总体重量持续增加。这表明在4至6个月时未发生明显的胸腺退化。胸腺切除术后，虽然低毒力病毒感染的发生率确实增加，但未观察到消瘦综合征。观察到循环淋巴细胞显著减少。使用全血培养法，用非特异性有丝分裂原、植物血凝素、细菌脂多糖和商陆有丝分裂原在体外刺激外周血淋巴细胞。观察结果包括夏季对植物血凝素和商陆有丝分裂原的反应更大，以及对所有有丝分裂原的反应随年龄变化。脂多糖刺激结果尚无定论。得出的结论是，新生犊牛胸腺切除术不是在牛种中产生选择性免疫抑制的令人满意的实验方法。
