Davis D R, Yeary R A, Randall G
Pediatr Pharmacol (New York). 1981;1(4):313-9.
Phototherapy has been widely used in the treatment of neonatal hyperbilirubinemia. Recent reports, however, have indicated that fluorescent light may be toxic and mutagenic to mammalian cells. these findings suggest possible long-term side effects with the use of phototherapy. This study was undertaken to determine the effects of phototherapy on hepatic microsomal enzyme activity. The exposure of Sprague-Dawley and Gunn rats to special blue fluorescent light at an average irradiance of 1,200 microW/cm2 resulted in no significant changes in liver microsomal enzyme activity for aniline hydroxylase, p-nitroanisole-O-demethylase, ethylmorphine-N-demethylase, cytochrome c reductase or the quantity of cytochrome P-450. A significant decrease in aniline hydroxylase and p-nitroanisole-O-demethylase activity was observed when liver microsomes were exposed in vitro to special blue fluorescent light. Photoactivated bilirubin did not effect the activity of the mixed-function oxidase enzymes measured under the conditions of this study.
光疗已广泛应用于新生儿高胆红素血症的治疗。然而，最近的报告表明，荧光灯可能对哺乳动物细胞有毒且具有致突变性。这些发现提示光疗可能存在长期副作用。本研究旨在确定光疗对肝微粒体酶活性的影响。将斯普拉格-道利大鼠和冈恩大鼠暴露于平均辐照度为1200微瓦/平方厘米的特殊蓝色荧光灯下，对苯胺羟化酶、对硝基苯甲醚-O-脱甲基酶、N-乙基吗啡脱甲基酶、细胞色素c还原酶或细胞色素P-450的量而言，肝微粒体酶活性未出现显著变化。当肝微粒体在体外暴露于特殊蓝色荧光灯下时，观察到苯胺羟化酶和对硝基苯甲醚-O-脱甲基酶活性显著降低。在本研究条件下，光活化胆红素对所测定的混合功能氧化酶的活性没有影响。
