Várkonyi T, Balint G A, Csáti S, Varrò V
Biochem Exp Biol. 1980;16(2):175-9.
A procedure is reported which is well applicable for the quantitative determination of glycogen, especially in cases of a small glycogen content in a small amount of tissue. The method consists of extraction of glycogen by boiling potassium hydroxide, precipitation with ethanol, hydrolysis with sulphuric acid, and determination of the glycogen in form of glucose obtained with orthotoluidine.
本文报道了一种非常适用于糖原定量测定的方法，尤其适用于少量组织中糖原含量较低的情况。该方法包括用氢氧化钾煮沸提取糖原、用乙醇沉淀、用硫酸水解，以及用邻甲苯胺测定以葡萄糖形式存在的糖原。
