Amgarten M, Schatzmann H J, Wüthrich A
J Vet Pharmacol Ther. 1981 Sep;4(3):241-8. doi: 10.1111/j.1365-2885.1981.tb00736.x.
In ruminal fluid, taken from a cow 2 h after cereal feeding and supplemented with powdered grass, chloral hydrate diminished the rate of acetate, butyrate and propionate production. Instead, a considerable increase in lactate production was elicited by the drug. Chloroform and trichloroethanol had the same effect. Chloral hydrate and chloroform rendered the redox potential more negative which indicates inhibition of methane production. A concentration of chloral hydrate (2 mM), giving complete methane inhibition, reduced volatile fatty acid production by 30%. The findings are explained by assuming that chloral hydrate blocks methane production but in addition is inhibitory at a step below lactate in the reductive pathway to propionate and that the conditions chosen resulted in substrate saturation of one of the enzymes in the succinate pathway to propionate.
在谷物喂食奶牛2小时后采集的瘤胃液中添加了粉状草，水合氯醛降低了乙酸盐、丁酸盐和丙酸盐的产生速率。相反，该药物引发了乳酸盐产生的显著增加。氯仿和三氯乙醇也有相同的效果。水合氯醛和氯仿使氧化还原电位变得更负，这表明甲烷产生受到抑制。2 mM浓度的水合氯醛可完全抑制甲烷产生，使挥发性脂肪酸的产生减少30%。这些发现的解释是，假定水合氯醛会阻碍甲烷产生，但此外在通向丙酸盐的还原途径中，在乳酸盐之下的一个步骤也有抑制作用，并且所选择的条件导致通向丙酸盐的琥珀酸盐途径中的一种酶出现底物饱和。
