Dukes P P, Ma A, Meytes D
Exp Hematol. 1980;8 Suppl 8:128-43.
Urinary proteins from patients with iron deficiency anemia and acquired aplastic anemia were fractionated by chromatography on QAE-Sephadex A-50 and Sephadex G-25. Fractions containing erythroid burst promoting activity (code named regulatory protein RP) were separated from erythropoietin. Mouse bone marrow cells were preincubated for one day in suspension culture, in the presence or absence of RP, transferred to a methylcellulose based system and incubated for six more days with erythropoietin (EPO). It was found that the presence of RP in the preincubation medium had a 2 to 4 fold enhancing effect on subsequent erythroid burst colony formation. However, when RP was added to methylcellulose based cultures simultaneously with EPO, the erythroid burst response was reduced or abolished. Addition of RP to marrow cell suspension cultures increased the number of self replicating, pluripotent (erythroid/granuloid, E/G ratio = 3) spleen colony forming units (CFU-S) found at the end of 2 days incubation 3-5 fold over their number in control cultures incubated without the factor. In marked contrast to this, addition of EPO to the cultures caused an increased persistence of CFU-S with a predominantly erythroid commitment (E/G ratio = 19) and a low self replicating ability, as measured by retransplantation of spleen cells into secondary recipients. These observations are compatible with the presence in RP of a factor, or factors, capable of maintaining the size of certain early precursor cell compartments.
采用QAE-葡聚糖凝胶A-50和葡聚糖凝胶G-25柱层析法对缺铁性贫血和获得性再生障碍性贫血患者的尿蛋白进行分离。从促红细胞生成素中分离出含有红系爆式促进活性（代号为调节蛋白RP）的组分。将小鼠骨髓细胞在有无RP的悬浮培养中预孵育一天，然后转移至基于甲基纤维素的体系中，并与促红细胞生成素（EPO）一起再孵育六天。结果发现，预孵育培养基中存在RP对随后的红系爆式集落形成有2至4倍的增强作用。然而，当RP与EPO同时添加到基于甲基纤维素的培养物中时，红系爆式反应降低或消失。向骨髓细胞悬浮培养物中添加RP，使在孵育2天结束时发现的自我复制、多能（红系/粒系，E/G比值 = 3）脾集落形成单位（CFU-S）的数量比未添加该因子的对照培养物中的数量增加了3至5倍。与此形成鲜明对比的是，向培养物中添加EPO会导致CFU-S的持续存在增加，其主要为红系定向（E/G比值 = 19）且自我复制能力较低，这通过将脾细胞重新移植到二级受体中进行测定。这些观察结果与RP中存在一种或多种能够维持某些早期前体细胞区室大小的因子相一致。
