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N-甲基-N'-硝基-N-亚硝基胍对体外DNA甲基化酶的抑制作用。

DNA methylase inhibition in vitro by N-methyl-N'-nitro-N-nitrosoguanidine.

作者信息

Cox R

出版信息

Cancer Res. 1980 Jan;40(1):61-3.

PMID:7349904
Abstract

Several alkylating carcinogens were tested for their ability to inhibit DNA methylation in an in vitro assay. N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) was the only carcinogen studied that altered the methylase activity. It was further demonstrated that MNNG reacts with the DNA methylase protein and produces a mixed type of inhibition. Prevention of the MNNG effect by dithiothreitol and inhibition of the DNA methylase with iodoacetamide suggest that DNA methylase is a sulfhydryl-containing enzyme and that MNNG inactivates the enzyme by reacting with sulfhydryl groups.

摘要

在一项体外试验中，对几种烷化致癌物抑制DNA甲基化的能力进行了测试。N-甲基-N'-硝基-N-亚硝基胍（MNNG）是所研究的唯一能改变甲基化酶活性的致癌物。进一步证明，MNNG与DNA甲基化酶蛋白发生反应，并产生混合型抑制作用。二硫苏糖醇可预防MNNG的作用，而碘乙酰胺可抑制DNA甲基化酶，这表明DNA甲基化酶是一种含巯基的酶，且MNNG通过与巯基反应使该酶失活。

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Cancer Res. 1980 Jan;40(1):61-3.
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Differential inhibition of rat liver DNA polymerases in vitro by direct-acting carcinogens and the protective effect of a thiol reducing agent.直接作用致癌物对大鼠肝脏DNA聚合酶的体外差异抑制作用及一种巯基还原剂的保护作用。
Biochem J. 1981 Mar 1;193(3):985-90. doi: 10.1042/bj1930985.
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