Nadler J V, Perry B W, Cotman C W
Brain Res. 1980 Jan 20;182(1):1-9. doi: 10.1016/0006-8993(80)90825-2.
Intraventricular injections of kainic acid were used to destroy the hippocampal CA3-CA4 cells, thus denervating the inner third of the molecular layer of the fascia dentata and stratum radiatum and stratum oriens of area CA1. The responses of intact afferents to such lesions were then examined histologically. The hippocampal mossy fibers densely reinnervated the inner portion of the dentate molecular layer after bilateral destruction of CA4 neurons and to a lesser extent after unilateral destruction. Septohippocampal fibers replaced CA4-derived fibers in the dentate molecular layer only after particularly extensive bilateral CA4 lesions. Medial perforant path fibers showed no anatomical response to any of these lesions. Neither septohippocampal, temporoammonic nor mossy fibers proliferated in or grew into the denervated laminae of area CA1. These results show a preferential ordering in the reinnervation of dentate granule cells which is not readily explained by proximity to the degenerating fibers and also that removal of CA3-CA4-derived innervation more readily elicits translaminar growth in the fascia dentata than in area CA1. These results may be relevant to clinical situations in which neurons of the hippocampal end-blade are lost.
向脑室内注射海藻酸以破坏海马CA3 - CA4细胞，从而使齿状回分子层内三分之一以及CA1区辐射层和原层去神经支配。然后通过组织学检查完整传入纤维对这种损伤的反应。在双侧破坏CA4神经元后，海马苔藓纤维密集地重新支配齿状分子层的内部，单侧破坏后程度较轻。仅在特别广泛的双侧CA4损伤后，隔海马纤维才在齿状分子层中取代源自CA4的纤维。内侧穿通路径纤维对这些损伤均无解剖学反应。隔海马纤维、颞叶-海马纤维和苔藓纤维均未在CA1区去神经支配的层中增殖或长入。这些结果表明，齿状颗粒细胞的重新支配存在优先顺序，这很难用与退变纤维的接近程度来解释，并且还表明，去除源自CA3 - CA4的神经支配比在CA1区更容易引发齿状回的跨层生长。这些结果可能与海马终叶神经元丢失的临床情况相关。
