Szymanovicz A, Joubert P M, Garbe A, Randoux A, Borel J P
Clin Chim Acta. 1980 Jan 15;100(2):155-64. doi: 10.1016/0009-8981(80)90077-7.
A fully automatic method permits fractionation in a Biogel P2 column of the hydroxyproline-containing peptides into two fractions. The alkaline hydrolysis and the colorimetric evaluation of the liberated hydroxyproline are also completely automatic and allow calculation of the percentages of the two fractions. The first one, termed F1 fraction, contains the peptides of molecular weight larger than 1500, while the other, termed F2, contains the smaller peptides. The method was used for 223 assays. The F1 fraction is decreased in cases of Paget's bone disease. It is increased in cases of metastatic cancer of bone. Statistical analysis of the data demonstrates that this techique greatly improves the certainty of diagnosis when coupled to the assay of total hydroxyproline. When both total urinary hydroxyproline and the F1 fraction percentage are increased over threshold values of 485 mumol per 24 h and 28.4% respectively, the probability of the presence of a bone metastasis is 100%.
一种全自动方法可将含羟脯氨酸的肽在Biogel P2柱中分离为两个组分。释放出的羟脯氨酸的碱性水解和比色评估也是完全自动的，并可计算两个组分的百分比。第一个组分称为F1组分，包含分子量大于1500的肽，而另一个组分称为F2，包含较小的肽。该方法用于223次检测。在佩吉特骨病病例中，F1组分减少。在骨转移性癌病例中，它会增加。对数据的统计分析表明，当与总羟脯氨酸检测相结合时，该技术大大提高了诊断的确定性。当尿总羟脯氨酸和F1组分百分比分别超过每24小时485微摩尔和28.4%的阈值时，骨转移存在的概率为100%。
