Dakkak R, Dreux C
Ann Biol Clin (Paris). 1976;34(3):197-202.
Urinary hydroxyproline is mainly in the form of oligopeptide and polypeptide, with a low proportion of free amino acid. Overall or partial estimation of hydroxyprolinuria may be carried out after isolation and hydrolysis of the peptides on ion exchange resin, by an automatic, continuous flow technique. This method presents a certain number of advantages, such as, selectivity, sensitivity and practicability, compared with that which we proposed in 1968. Determination of the criteria of quality of the method shows that it is suitable for exploration of variations in hydroxyprolinuria in diseases of bone and collagen. The reference values in relation to age and sex are also reported.
尿羟脯氨酸主要以寡肽和多肽的形式存在，游离氨基酸的比例较低。可通过离子交换树脂上肽的分离和水解，采用自动连续流动技术对羟脯氨酸尿症进行整体或部分评估。与我们1968年提出的方法相比，该方法具有一定的优势，如选择性、灵敏度和实用性。对该方法质量标准的测定表明，它适用于探索骨和胶原蛋白疾病中羟脯氨酸尿症的变化。还报告了与年龄和性别相关的参考值。
