使用 Percoll 分离甲烷营养菌及其在混合和纯培养物分离中的应用。
Separation of methanotrophic bacteria by using percoll and its application to isolation of mixed and pure cultures.
机构信息
Center for Great Lakes Studies and Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, and Keck Laboratories, 138-78 California Institute of Technology, Pasadena, California 91125.
出版信息
Appl Environ Microbiol. 1991 Dec;57(12):3656-9. doi: 10.1128/aem.57.12.3656-3659.1991.
Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.
I 型和 II 型甲烷营养菌可以通过使用 Percoll 等密度梯度法进行清晰分离。这种简单且廉价的方法可用于快速筛选新的分离物，进行初步分类。此外，Percoll 梯度还可用于增强从天然水样中富集甲烷营养菌的标准技术。
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