Allan M, Harrison P
Cell. 1980 Feb;19(2):437-47. doi: 10.1016/0092-8674(80)90518-8.
We have studied the regulation of differentiation within the hemopoietic system by fusing mouse Friend cells (which can be induced to undergo red blood cell differentiation) to various mouse lymphomas and myelomas which express characteristic T and B lymphocytes surface antignes. Our results show that both erythroid and lymphoid differentiation markers can be co-expressed within the same cell. To determine whether this result applies to other differentiation states, we fused suspension Friend cells to three adherent fibroblast cell lines, and isolated both adherent and suspension hybrids. In fact, suspension hybrid clones were inducible for hemoglobin, whereas adherent clones were not. No obvious differences in overall chromosome balance were evident between the adherent and suspension hybrids. A similar correlation between suspension morphology and inducibility of hemoglobin was found in hybrids between suspension Friend cells and an adherent lymphoma line. These results show that different developmental programs can be co-expressed within the same hybrid cell; but the strongly adherent type of morphology is inconsistent with expression of the red blood cell phenotype, both in hybrid cells derived entirely from hemopoietic parental cells and in cells from widely different lineages.
我们通过将小鼠弗瑞德细胞（可被诱导进行红细胞分化）与各种表达特征性T和B淋巴细胞表面抗原的小鼠淋巴瘤和骨髓瘤细胞融合，研究了造血系统内分化的调控。我们的结果表明，红细胞和淋巴细胞分化标志物可在同一细胞中共表达。为了确定这一结果是否适用于其他分化状态，我们将悬浮的弗瑞德细胞与三种贴壁成纤维细胞系融合，并分离出贴壁和悬浮杂种细胞。事实上，悬浮杂种克隆可被诱导产生血红蛋白，而贴壁克隆则不能。贴壁和悬浮杂种细胞之间在总体染色体平衡上没有明显差异。在悬浮的弗瑞德细胞与一种贴壁淋巴瘤细胞系的杂种细胞中，也发现了悬浮形态与血红蛋白诱导能力之间的类似相关性。这些结果表明，不同的发育程序可在同一杂种细胞中共表达；但无论是在完全源自造血亲本细胞的杂种细胞中，还是在源自广泛不同谱系的细胞中，强贴壁型形态都与红细胞表型的表达不一致。
