Lee H K, Dunwiddie T, Hoffer B
Brain Res. 1980 Feb 24;184(2):331-42. doi: 10.1016/0006-8993(80)90802-1.
The effects of active and inactive enkephalin derivatives and naloxone on putative interneurons were studied in the in vitro hippocampal slice. Inhibitory interneurons were recorded from extracellularly, and identified electrophysiologically on the basis of their characteristic action potential shape and pattern of evoked firing in response to single and multiple electrical stimuli. Active enkephalin derivatives elicited a dose-dependent depression in excitability whereas inactive derivatives had no effect. Naloxone reliably and reproducibly antagonized the depressant action of active enkephalins. These data confirm the hypothesis outlined in the preceding communication, that the direct effect of enkephalins in the hippocampus is a depression of firing of inhibitory neurons, and support the hypothesis that enkephalin-induced excitations of pyramidal cells are brought about by disinhibition.
在体外海马切片中研究了活性和非活性脑啡肽衍生物以及纳洛酮对假定中间神经元的作用。从细胞外记录抑制性中间神经元，并根据其特征性动作电位形状和对单次和多次电刺激诱发放电的模式进行电生理鉴定。活性脑啡肽衍生物引起兴奋性呈剂量依赖性降低，而非活性衍生物则无作用。纳洛酮可靠且可重复地拮抗活性脑啡肽的抑制作用。这些数据证实了前一篇通讯中概述的假设，即脑啡肽在海马体中的直接作用是抑制抑制性神经元的放电，并支持脑啡肽诱导的锥体细胞兴奋是由去抑制引起的这一假设。
