通过凝胶过滤法纯化桦树花粉变应原提取物。I. 该组分的化学和免疫学特性

Purification of birch pollen allergen extract by gel filtration. I. Chemical and immunological characterization of the fraction.

作者信息

Puttonen E, Pilström L

出版信息

Int Arch Allergy Appl Immunol. 1980;61(3):299-307. doi: 10.1159/000232452.

Abstract

Birch pollen extract was fractionated by gel filtration on a Sephadex G-75 column. The physicochemical characterization of fractions included the determination of protein, carbohydrate, molecular weight and pI. The immunological properties of the fractions were measured by skin prick testing of allergenic individuals and by indirect RAST. The cross-reactivity of the fractions was investigated by heterologous PCA in rats after producing specific reaginic sera in mice. The allergenically most active fractions were composed mainly of proteins with a molecular weight range of 10,000--50,000 daltons. These active fractions represented only 3% of the total protein and carbohydrate content of the crude extract. These results indicate that it is possible to purify birch pollen allergen extract from the bulk of nonallergic contaminants, mainly low molecular weight carbohydrates, by a single step gel filtration procedure.

摘要

桦树花粉提取物通过在葡聚糖G - 75柱上进行凝胶过滤进行分级分离。各分级部分的物理化学特性包括蛋白质、碳水化合物、分子量和等电点的测定。通过对过敏个体进行皮肤点刺试验以及间接放射变应原吸附试验（RAST）来测定各分级部分的免疫学特性。在小鼠体内产生特异性反应素血清后，通过大鼠体内的异种被动皮肤过敏试验（PCA）研究各分级部分的交叉反应性。过敏原活性最高的分级部分主要由分子量范围为10,000 - 50,000道尔顿的蛋白质组成。这些活性分级部分仅占粗提物总蛋白质和碳水化合物含量的3%。这些结果表明，通过单步凝胶过滤程序，有可能从大量非过敏污染物（主要是低分子量碳水化合物）中纯化桦树花粉过敏原提取物。