Wahl R, Schmid-Grendelmeier P, Cromwell O, Wüthrich B
Allergopharma, Joachim Ganzer KG, Reinbek, Germany.
J Allergy Clin Immunol. 1996 Jul;98(1):99-106. doi: 10.1016/s0091-6749(96)70231-2.
Allergenic cross-reactivity between members of the Fagales family (birch, alder, hazel, and beech) and between members of the Oleaceae family (ash, olive, lilac, and privet) is well known, but little is known about possible cross-reactivity between these two groups of trees, in particular between birch and ash, both of which flower in the spring. Various immunochemical methods including RAST inhibition, Western blot, and Western blot inhibition have been used in this study to show that there is partial cross-reactivity between birch and ash pollens. Enzyme allergosorbent test measurements were performed on sera from 35 patients with hay fever in spring by using birch and ash pollen allergen disks. The major allergen of birch, Bet v 1, was readily detectable in the birch pollen extract, but a homologous allergen in the ash pollen extract was barely detectable. Common allergens could be determined in the high molecular weight region. Ash pollen should be included in diagnostic procedures for spring pollinosis and should be considered for use in specific immunotherapy.
壳斗目植物（桦树、桤木、榛树和山毛榉）之间以及木犀科植物（白蜡树、橄榄树、丁香和女贞）之间的变应原交叉反应是众所周知的，但对于这两组树木之间可能存在的交叉反应，尤其是在春季开花的桦树和白蜡树之间的交叉反应，人们却知之甚少。本研究采用了包括放射性变应原吸附试验抑制、蛋白质免疫印迹法和蛋白质免疫印迹抑制在内的各种免疫化学方法，以表明桦树花粉和白蜡树花粉之间存在部分交叉反应。通过使用桦树和白蜡树花粉变应原盘，对35名春季花粉症患者的血清进行了酶联变应吸附试验测量。桦树的主要变应原Bet v 1在桦树花粉提取物中很容易检测到，但在白蜡树花粉提取物中几乎检测不到同源变应原。在高分子量区域可以确定常见变应原。白蜡树花粉应纳入春季花粉症的诊断程序，并应考虑用于特异性免疫治疗。
