Zalman L S, Nikaido H, Kagawa Y
J Biol Chem. 1980 Mar 10;255(5):1771-4.
Vesicles permeable to sucrose, but not to high molecular weight dextrans, were produced when fragments of mitochondrial outer membrane obtained from rat liver or mung bean seedlings were fused with liposomes of soybean phospholipids. By using this reconstitution assay, the channel-forming protein in the mung bean mitochondria was identified as protein(s) with an apparent molecular weight of 30,000 through differential detergent extraction and centrifugation in a sucrose density gradient. The channel was nonspecific, and allowed the diffusion of saccharides of up to 2000 to 8000 daltons.
当从大鼠肝脏或绿豆幼苗中获得的线粒体外膜片段与大豆磷脂脂质体融合时，会产生对蔗糖可渗透但对高分子量葡聚糖不可渗透的囊泡。通过使用这种重组测定法，绿豆线粒体中的通道形成蛋白通过在蔗糖密度梯度中进行差异去污剂提取和离心，被鉴定为表观分子量为30,000的一种或多种蛋白质。该通道是非特异性的，允许分子量高达2000至8000道尔顿的糖类扩散。
