de Vries J E, Mendelsohn J, Bont W S
Nature. 1980 Feb 7;283(5747):574-6. doi: 10.1038/283574a0.
Human lymphocytes have been shown to lyse in vitro a large variety of target cells derived from tumour as well as normal tissues. The effector cells involved in this spontaneous cell-mediated cytotoxicity (SCMC) have generally been characterised as lymphocytes which lack surface membrane immunoglobulin (sIg) and lack or express only low-affinity receptors for sheep red blood cells (SRBC), but possess receptors for the Fc portion of IgG (refs 3--7). We have previously shown that monocytes may be involved in SCMC, as strong depletions of adherent cells always resulted in a reduction in SCMC. In the present study, experiments were undertaken to determine the role of monocytes in SCMC. Evidence is presented that monocytes have a helper function in the lymphocyte-mediated SCMC against target cells growing in monolayer cultures, whereas effector cells in SCMC against lymphoid target cells growing in suspension cultures were lysed by lymphocytes in the absence of monocytes.
已证实，人类淋巴细胞在体外可溶解多种来源于肿瘤组织和正常组织的靶细胞。参与这种自发细胞介导的细胞毒性作用（SCMC）的效应细胞通常被界定为缺乏表面膜免疫球蛋白（sIg）、对绵羊红细胞（SRBC）缺乏或仅表达低亲和力受体，但拥有针对IgG的Fc部分的受体的淋巴细胞（参考文献3 - 7）。我们之前已表明，单核细胞可能参与SCMC，因为贴壁细胞的大量缺失总会导致SCMC降低。在本研究中，我们进行了实验以确定单核细胞在SCMC中的作用。有证据表明，单核细胞在淋巴细胞介导的针对单层培养中生长的靶细胞的SCMC中具有辅助功能，而在针对悬浮培养中生长的淋巴样靶细胞的SCMC中，效应细胞在无单核细胞的情况下会被淋巴细胞裂解。
