Kohl S, Pickering L K, Loo L S
Infect Immun. 1982 May;36(2):691-5. doi: 10.1128/iai.36.2.691-695.1982.
Natural killer cytotoxicity is an important antiviral defense mechanism. Human peripheral blood mononuclear cells cultured with herpes simplex virus (HSV)-infected cells produced a cytokine. This substance stimulated adult natural killer cytotoxicity from 53.0 +/- 10.5% to 79.8% (P less than 0.01) against HSV-infected target cells. These data resulted in a calculated cytokine-dependent cellular cytotoxicity (CDCC) value of 65.8%. Cytokine production was not stimulated by uninfected cells and was independent of the presence or absence of antibodies to HSV in sera of donors and mononuclear cells. Cells from human colostrum also produced an HSV-stimulated cytokine which mediated CDCC by using both adult (19.8 +/- 3.9%) and neonatal (18.6 +/- 3.4%) mononuclear effectors cells. Colostral cell cytokine production was also independent of donor HSV serology. Not all colostral cultures produced the cytokine, and in general colostrum-stimulated CDCC was lower than peripheral blood leukocyte-stimulated CDCC. Colostral cell cytokine stimulation of neonatal natural killer cytotoxicity may account in part for the increased nonspecific resistance of breast-fed infants to viral infection.
自然杀伤细胞的细胞毒性是一种重要的抗病毒防御机制。用单纯疱疹病毒（HSV）感染的细胞培养人外周血单核细胞可产生一种细胞因子。该物质可使成人针对HSV感染靶细胞的自然杀伤细胞毒性从53.0±10.5%提高至79.8%（P＜0.01）。这些数据得出的细胞因子依赖性细胞毒性（CDCC）值为65.8%。未感染的细胞不会刺激细胞因子的产生，且细胞因子的产生与供体血清和单核细胞中是否存在抗HSV抗体无关。人初乳中的细胞也会产生一种受HSV刺激的细胞因子，该细胞因子通过使用成人（19.8±3.9%）和新生儿（18.6±3.4%）单核效应细胞介导CDCC。初乳细胞产生细胞因子也与供体的HSV血清学无关。并非所有初乳培养物都会产生细胞因子，总体而言，初乳刺激的CDCC低于外周血白细胞刺激的CDCC。初乳细胞因子对新生儿自然杀伤细胞毒性的刺激可能部分解释了母乳喂养婴儿对病毒感染的非特异性抵抗力增强的原因。
