Carpentieri U, Sordahl L A
Cancer Res. 1980 Feb;40(2):221-4.
Methods have been developed to isolate mitochondria from small amounts of normal and leukemic human lymphocytes obtained from small volumes of circulating blood. Mitochondrial respiratory functions were measured by polarographic techniques, and active calcium uptake was measured by a spectrophotometric procedure utilizing the calcium-sensitive dye murexide. The results indicate that the rates of active oxygen consumption of normal human lymphocyte mitochondria are very low in comparison to values obtained from a number of animal tissue sources. Mitochondria extracted from the lymphocytes of leukemic patients and from cultured, and phytohemagglutinin-transformed human lymphocytes exhibited higher respiratory rates than did normal controls. Although respiratory rates were relatively low in all preparations, the efficiency of energy coupling (ADP:O, ratio of nmol of adenosine diphosphate phosphorylated to nanoatoms of O2 consumed) was within normal limits. The mitochondria extracted from leukemia, cultured, and phytohemagglutinin-transformed lymphocytes exhibited high rates of respiratory substrate-supported calcium uptake compared to controls.
已经开发出从少量取自小体积循环血液的正常和白血病人类淋巴细胞中分离线粒体的方法。通过极谱技术测量线粒体呼吸功能，并通过利用钙敏染料骨螺紫的分光光度法测量活性钙摄取。结果表明，与从多种动物组织来源获得的值相比，正常人淋巴细胞线粒体的活性氧消耗率非常低。从白血病患者的淋巴细胞以及培养的和经植物血凝素转化的人类淋巴细胞中提取的线粒体表现出比正常对照更高的呼吸速率。尽管所有制剂中的呼吸速率相对较低，但能量偶联效率（ADP:O，即磷酸化的二磷酸腺苷的纳摩尔数与消耗的氧的纳米原子数之比）在正常范围内。与对照相比，从白血病、培养的和经植物血凝素转化的淋巴细胞中提取的线粒体表现出高呼吸底物支持的钙摄取率。
