Medina D, Oborn C J, Asch B B
Cancer Res. 1980 Feb;40(2):329-33.
Epithelial cell cultures of normal mammary gland, preneoplastic hyperplastic nodule outgrowth lines, primary tumors, and transplanted tumors, all derived from BALB/c mice, were examined for their response to cytochalasin B to determine if cells of primary mammary tumors multinucleated and if preneoplastic hyperplastic alveolar nodule cells responded differently than cells of primary tumors. Established tumorigenic and nontumorigenic cell lines were also examined as positive and negative controls. The standard assay conditions were optimized at 1 microgram CB per ml for 48 hr. The results, expressed as the mean percentage of cells exhibiting three or more nuclei per cell were: normal mammary cells, 5%; preneoplastic mammary cells, 4%; primary mammary tumor cells, 36%; transplanted mammary tumors, 70%; tumorigenic established cell lines, 80%; and nontumorigenic established cell lines, 5%. The frequency of tumor cells exhibiting multinucleation increased with serial transplantation in vivo and with serial passage in vitro. The results demonstrate that neoplastic cells within a primary tumor exhibit uncontrolled nuclear division and that uncontrolled nuclear division is a distinguishing characteristic between preneoplastic and neoplastic mammary cells.
对所有来源于BALB/c小鼠的正常乳腺上皮细胞培养物、癌前增生性结节生长系、原发性肿瘤和移植性肿瘤，检测它们对细胞松弛素B的反应，以确定原发性乳腺肿瘤细胞是否多核，以及癌前增生性肺泡结节细胞与原发性肿瘤细胞的反应是否不同。已建立的致瘤和非致瘤细胞系也作为阳性和阴性对照进行检测。标准检测条件优化为每毫升1微克细胞松弛素B作用48小时。结果以每个细胞出现三个或更多细胞核的细胞平均百分比表示：正常乳腺细胞为5%；癌前乳腺细胞为4%；原发性乳腺肿瘤细胞为36%；移植性乳腺肿瘤为70%；致瘤性已建立细胞系为80%；非致瘤性已建立细胞系为5%。肿瘤细胞多核化的频率随体内连续移植和体外连续传代而增加。结果表明，原发性肿瘤内的肿瘤细胞表现出不受控制的核分裂，并且不受控制的核分裂是癌前和肿瘤性乳腺细胞之间的一个显著特征。
