Kumar A, Padmanaban G
J Biol Chem. 1980 Jan 25;255(2):522-5.
The synthesis of cytochrome P-450 (phenobarbital inducible) and cytochrome P-448 (3-methylcholanthrene inducible) have been studied in rat liver in vivo and in the wheat germ cell-free system using anti-cytochrome P-450 and anti-cytochrome P-448 antibodies. The major mature forms synthesized in vivo correspond to a molecular weight of 47,000 for cytochrome P-450 and 53,000 for cytochrome P-448. Translation of poly(A)-containing RNA from phenobarbital-treated rats in the wheat germ cell-free system reveals that the cell-free product immunoprecipitated with anti-cytochrome P-450 antibody has a molecular weight close to 47,000. In the case of 3-methylcholanthrene, the cell-free product immunoprecipitated with anti-cytochrome P-448 antibody shows a molecular weight around 59,000. Significant conversion of the 59,000 species to the 53,000 species can be demonstrated when the translation is carried out in the presence of microsomal membranes isolated from rat liver. Phenobarbital and 3-methylcholanthrene enhance the translatable messenger RNA contents for cytochrome P-450 and cytochrome P-448, respectively.
利用抗细胞色素P - 450和抗细胞色素P - 448抗体，在大鼠肝脏体内以及小麦胚芽无细胞体系中，对细胞色素P - 450（苯巴比妥诱导型）和细胞色素P - 448（3 - 甲基胆蒽诱导型）的合成进行了研究。体内合成的主要成熟形式中，细胞色素P - 450的分子量为47,000，细胞色素P - 448的分子量为53,000。在小麦胚芽无细胞体系中翻译经苯巴比妥处理的大鼠的含poly(A) RNA，结果显示，用抗细胞色素P - 450抗体免疫沉淀的无细胞产物分子量接近47,000。对于3 - 甲基胆蒽，用抗细胞色素P - 448抗体免疫沉淀的无细胞产物分子量约为59,000。当在存在从大鼠肝脏分离的微粒体膜的情况下进行翻译时，可以证明59,000的物种会大量转化为53,000的物种。苯巴比妥和3 - 甲基胆蒽分别提高了细胞色素P - 450和细胞色素P - 448的可翻译信使RNA含量。
